Description
Gluten ELISA Ordering Information
| Cat Code. | Method | Assay Time | Limit of Detection (LoD) | Sample Types |
| GLU-EK-96
GLU-EK-48 |
ELISA 96T
ELISA 48T |
30 mins (post extraction) | 0.36ppm (0.18ppm gliadin) | Cereals
Tree Nuts Legumes Seeds Meat Spices |
The MonoTrace Gluten ELISA kit provides highly sensitive and reliable detection of gluten in food products and on production surfaces. Designed for food manufacturers, laboratories, and quality control teams, it ensures compliance with gluten-free labelling regulations and supports the highest standards of food safety.
This kit uses a single-step, non-toxic extraction method, streamlining testing while eliminating hazardous waste, making gluten testing faster, safer, and more environmentally sustainable. It accurately detects gluten contamination from wheat, barley, and rye across a wide range of food products, including baked goods, cereals, snacks, sauces, beverages, and processed foods, with minimal cross-reactivity.
The MonoTrace Gluten ELISA also supports surface swab testing to verify cleaning effectiveness and prevent cross-contamination, aiding compliance with Good Manufacturing Practice (GMP).
Manufactured in an ISO 13485-accredited facility, the MonoTrace Gluten ELISA kit delivers rapid, reproducible, and dependable results—supporting robust allergen management, food safety assurance, and the protection of brand reputation.
- Extraction Time: 1 hour
- Testing Time: 30 minutes
- Specificity: Gliadin
- Limit of Detection: < 1 ppm
- Range of Quantification: 2-100 ppm
- New Monoclonal Antibodies (Mabs):
Anti-gliadin Mab-coated assay plate
HRP-labeled anti-gliadin Mab conjugate - Assay Plate: Breakaway microwell strips
- Extraction:
Non-toxic extraction buffer
Single step (one buffer, one temperature) - Ready-to-Use Standards: 0 ppm, 2 ppm, 10 ppm, 20 ppm,
40 ppm, and 100 ppm gluten controls included
Validated for use with various raw and processed foods via proficiency testing and reference material analyses, the BioFront Technologies MonoTrace Gluten ELISA represents a new approach for the quantitative or qualitative detection of gluten. This ELISA kit uses a new
pair of highly sensitive monoclonal antibodies to specifically target and detect the α-gliadin component of wheat and homologous proteins in barley (hordein) and rye (secalin).
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Gluten ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable Almond allergen resistant to food processing effects
- Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
- Perform well in a wide variety of food products with negligible food matrix interference
