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The Gluten ELISA kit is a novel sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative or qualitative detection of gluten in food samples. The kit uses a novel pair of highly sensitive monoclonal antibodies to specifically target and detect the α-gliadin component of gluten. The Gluten ELISA kit incorporates a new single-step non-toxic extraction method (patent pending) resulting in a highly sensitive and specific method for the detection of gluten in raw ingredients and processed foods. The United States Food and Drug Administration (FDA) and Codex Alimentarius states that foods declared as gluten-free must contain less than 20 parts per million (ppm) gluten.