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Home  /  Products  /  Allergens, Dried Fruit & Nuts, Others  /  Lupin ELISA

Lupin ELISA

The MonoTrace Lupin ELISA kit can be used to detect and quantify the presence of lupin protein in complex food matrices or to check for the removal of potentially allergenic lupin protein residues after surface swabbing.

Lupin (lupine), a legume belonging to the same plant family as peanuts, can serve as a rich source of protein and is being increasingly used in bakery, confectionery, snacks and pastry products world wide due to its multifunctionality. The popularity of lupin as a food ingredient has experienced rapid grow due, in part, to its use as a substitute for gluten-containing flours in gluten-free products. In Europe, where lupin has long been widely used in food products, there has been mandatory allergen labeling for food products containing lupin since 2007 and, in 2016, the Food Standards agency for Australia and New Zealand announced that they were “assessing if lupin should be regulated as food allergen.” BioFront’s MonoTrace Lupin ELISA kit is best-in-class for lupin detection in foods and beverages and on surfaces.

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Description

Lupin ELISA Ordering Information

Cat Code. Method Assay Time Limit of Detection (LoD) Sample Types
LU2-EK-96

LU2-EK-48

 ELISA 96T

ELISA 48T

 30 mins (post extraction) 0.13ppm Flour (wheat, rice, oat, chickpea, lentil)

Chocolate

Orange Juice

Wine

Ketchup

Crackers

Fruit & Nut Mix

Baby Food

Raw Meat

Red Onion

Seasoning

Bread/Cake/Cookie Mixes

Ice Cream

Product Description

The Biorex Food Diagnostics MonoTrace Lupin ELISA kit provides highly sensitive and specific detection of lupin protein in food products and on production surfaces. It is designed for food manufacturers, laboratories, and quality control teams. In addition, it supports compliance with allergen labelling regulations and strengthens overall allergen management systems.

This ELISA test accurately detects lupin contamination in complex food matrices such as baked goods, confectionery, pastries, snacks, and gluten-free products. Furthermore, it supports surface swab testing to verify cleaning effectiveness and reduce the risk of cross-contamination. As a result, it helps manufacturers maintain Good Manufacturing Practice (GMP) compliance and improve production hygiene controls.

Manufactured in an ISO 13485-accredited facility, the MonoTrace Lupin ELISA kit delivers rapid, reproducible, and dependable results. Moreover, strict quality control during production ensures consistent performance across batches. Consequently, laboratories and manufacturers can rely on robust data for routine testing and decision-making.

Overall, the MonoTrace Lupin ELISA kit supports effective allergen management, enhances food safety assurance, and helps protect brand reputation by ensuring accurate detection of lupin allergens across the food supply chain.

Key Benefits

  • Target major food allergens
  • Robust assay with high specificity to target allergen
  • Quantitative assay in 30 minutes
  • Uniform extraction for multiple allergens
  • Wide quantitative range (e.g., 1 – 40 ppm)
  • Reduce food matrix interference and cross-reactivity
  • Sensitivity at or below 1 ppm
  • Ready-to-use Standards

MonoTrace® ELISA Kits

MonoTrace ELISA kits represent the first comprehensive line of monoclonal antibody-based assays designed to help the food industry accurately detect and quantify trace amounts of nut allergens in various food matrices. In addition, these ELISAs incorporate precisely defined sets of monoclonal antibodies that ensure high specificity and reliable performance across complex food systems.

Furthermore, they include monoclonal antibodies targeting major food allergens such as almond. As a result, the kits deliver reliable quantitative results in just 30 minutes post-extraction, thanks to three 10-minute incubation steps.

  • 10-minute sample incubation
  • 10-minute conjugate incubation
  • 10-minute substrate incubation

The MonoTrace® Advantage

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. In particular, the MonoTrace Lupin ELISA incorporates a precisely defined set of handpicked monoclonal antibodies. These were selected after a rigorous screening process for their ability to:

  • Target a highly stable lupin allergen resistant to food processing effects
  • Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
  • Perform well on a wide variety of food products with negligible food matrix interference

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