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Home  /  Products  /  Allergens, Dried Fruit & Nuts, Others  /  Lupin ELISA


The MonoTrace Lupin ELISA kit can be used to detect and quantify the presence of lupin protein in complex food matrices or to check for the removal of potentially allergenic lupin protein residues after surface swabbing.

Lupin (lupine), a legume belonging to the same plant family as peanuts, can serve as a rich source of protein and is being increasingly used in bakery, confectionery, snacks and pastry products world wide due to its multifunctionality. The popularity of lupin as a food ingredient has experienced rapid grow due, in part, to its use as a substitute for gluten-containing flours in gluten-free products. In Europe, where lupin has long been widely used in food products, there has been mandatory allergen labeling for food products containing lupin since 2007 and, in 2016, the Food Standards agency for Australia and New Zealand announced that they were “assessing if lupin should be regulated as food allergen.” BioFront’s MonoTrace Lupin ELISA kit is best-in-class for lupin detection in foods and beverages and on surfaces.

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Lupin ELISA Ordering Information

Cat Code. Method Assay Time Limit of Detection (LoD) Sample Types




30 mins (post extraction) 0.13ppm Flour (wheat, rice, oat, chickpea, lentil)


Orange Juice




Fruit & Nut Mix

Baby Food

Raw Meat

Red Onion


Bread/Cake/Cookie Mixes

Ice Cream

• Target major food allergens
• Robust assay with high specificity to target allergen
• Quantitative assay in 30 minutes
• Uniform extraction for multiple allergens
• Wide quantitative range (e.g., 1 – 40 ppm)
• Reduce food matrix interference and cross-reactivity
• Sensitivity at or below 1 ppm
• Ready-to-use Standards

MonoTrace ELISA kits represent the first comprehensive line of monoclonal antibody-based assays aimed at helping the food industry to accurately detect and quantify trace amounts of nut allergens in various food matrices. These ELISAs incorporate precisely defined sets
of monoclonal antibodies targeting major food allergens such as almond and provide quantitative results in just 30minutes post-extraction thanks to three 10-minute incubations!
• 10-minute sample incubation
• 10-minute conjugate incubation
• 10-minute substrate incubation

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Lupin ELISA incorporates a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

  • Target a highly stable lupin allergen resistant to food processing effects
  • Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
  • Perform well on a wide variety of food products with negligible food matrix interference