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Pecan ELISA

The MonoTrace Pecan ELISA kit can be used to detect and quantify the presence of pecan protein in complex food matrices or to check for the removal of potentially allergenic pecan protein residues after surface swabbing.

Pecans are grown extensively in the Southern US, and BioFront’s MonoTrace ELISA kit is recognized as the best-in-class detection method for Pecan. Allergic reactions to Pecan typically last a lifetime, so its accurate detection in foods is of the utmost importance.

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Pecan ELISA Ordering Information

Cat Code. Method Assay Time Limit of Detection (LoD) Sample Types
PC4-EK-96

PC4-EK-48

ELISA 96T

ELISA 48T

30 mins (post extraction) 0.17ppm Cake Mix

Cookies

Flours

Chocolate

Infant Soy Formula

Yoghurt

Ice-Cream

The MonoTrace Pecan ELISA kit provides highly sensitive and reliable detection of pecan protein in food products and on production surfaces. Designed for food manufacturers, laboratories, and quality control teams, it ensures compliance with allergen labelling regulations and helps protect consumers with pecan allergies.

This ELISA test accurately detects pecan contamination in complex food matrices, including baked goods, confectionery, snacks, desserts, and processed foods. It supports both quantitative measurement and qualitative screening, offering high specificity with minimal cross-reactivity. The kit also enables surface swab testing to verify cleaning effectiveness and prevent cross-contamination, supporting Good Manufacturing Practice (GMP).

Manufactured in an ISO 13485-accredited facility, the MonoTrace Pecan ELISA kit delivers rapid, reproducible, and dependable results—supporting robust allergen management, food safety assurance, and protection of brand reputation.

• Target major food allergens
• Robust assay with high specificity to target allergen
• Quantitative assay in 30 minutes
• Uniform extraction for multiple allergens
• Wide quantitative range (e.g., 1 – 40 ppm)
• Reduce food matrix interference and cross-reactivity
• Sensitivity at or below 1 ppm
• Ready-to-use Standards

MonoTrace ELISA kits represent the first comprehensive line of monoclonal antibody-based assays aimed at helping the food industry to accurately detect and quantify trace amounts of nut allergens in various food matrices. These ELISAs incorporate precisely defined sets
of monoclonal antibodies targeting major food allergens such as almond and provide quantitative results in just 30minutes post-extraction thanks to three 10-minute incubations!
• 10-minute sample incubation
• 10-minute conjugate incubation
• 10-minute substrate incubation

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Pecan ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

  • Target a highly stable Pecan allergen resistant to food processing effects
  • Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
  • Perform well in a wide variety of food products with negligible food matrix interference