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The Macadamia nut ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative or qualitative detection of macadamia nut. This monoclonal antibody (MAb)-based assay provides a highly sensitive and specific method for the quantification of macadamia nut presence/contamination within a variety of food matrices. It may also be used for simple “yes-no” qualitative assessments. The kit enables a facile and quantitative measure of macadamia without the cross-reactivity issues often associated with polyclonal antibody (PAb)-based ELISA kits. The target indicator protein is a major macadamia allergen and was selected based on its strong resistance to food processing and high abundance in the nut.