Description
Walnut ELISA Ordering Information
| Cat Code. | Method | Assay Time | Limit of Detection (LoD) | Sample Types |
| WJ4-EK-96
WJ4-EK-48 |
ELISA 96T
ELISA 48T |
30 mins (post extraction) | 0.2ppm | Cereal
Cake Flour Ice Cream Infant Soy Formula Chocolate Cookies |
The MonoTrace Walnut ELISA kit offers highly sensitive and reliable detection of walnut protein in food products and on production surfaces. Designed for food manufacturers, laboratories, and quality control teams, it ensures compliance with allergen labelling regulations and protects consumers with walnut allergies.
This ELISA test accurately detects walnut contamination in complex food matrices, including baked goods, confectionery, desserts, snacks, and processed meals. It provides both quantitative measurement and qualitative “yes/no” screening, with high specificity to walnuts and minimal cross-reactivity with other tree nuts. The kit also supports surface swab testing to verify cleaning effectiveness and prevent cross-contamination, aligning with Good Manufacturing Practice (GMP).
Manufactured in an ISO 13485-accredited facility, the MonoTrace Walnut ELISA kit delivers rapid, reproducible, and dependable results—supporting robust allergen management, food safety compliance, and protection of brand reputation.
• Target major food allergens
• Robust assay with high specificity to target allergen
• Quantitative assay in 30 minutes
• Uniform extraction for multiple allergens
• Wide quantitative range (e.g., 1 – 40 ppm)
• Reduce food matrix interference and cross-reactivity
• Sensitivity at or below 1 ppm
• Ready-to-use Standards
MonoTrace ELISA kits represent the first comprehensive line of monoclonal antibody-based assays aimed at helping the food industry to accurately detect and quantify trace amounts of nut allergens in various food matrices. These ELISAs incorporate precisely defined sets
of monoclonal antibodies targeting major food allergens such as almond and provide quantitative results in just 30minutes post-extraction thanks to three 10-minute incubations!
• 10-minute sample incubation
• 10-minute conjugate incubation
• 10-minute substrate incubation
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Walnut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable Walnut allergen resistant to food processing effects
- Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
- Perform well in a wide variety of food products with negligible food matrix interference
