Description
Gluten ELISA Ordering Information
| Cat Code. | Method | Assay Time | Limit of Detection (LoD) | Sample Types |
| GLU-EK-96
GLU-EK-48 |
ELISA 96T
ELISA 48T |
30 mins (post extraction) | 0.36ppm (0.18ppm gliadin) | Cereals
Tree Nuts Legumes Seeds Meat Spices |
- Extraction Time: 1 hour
- Testing Time: 30 minutes
- Specificity: Gliadin
- Limit of Detection: < 1 ppm
- Range of Quantification: 2-100 ppm
- New Monoclonal Antibodies (Mabs):
Anti-gliadin Mab-coated assay plate
HRP-labeled anti-gliadin Mab conjugate - Assay Plate: Breakaway microwell strips
- Extraction:
Non-toxic extraction buffer
Single step (one buffer, one temperature) - Ready-to-Use Standards: 0 ppm, 2 ppm, 10 ppm, 20 ppm,
40 ppm, and 100 ppm gluten controls included
Validated for use with various raw and processed foods via proficiency testing and reference material analyses, the BioFront Technologies MonoTrace Gluten ELISA represents a new approach for the quantitative or qualitative detection of gluten. This ELISA kit uses a new
pair of highly sensitive monoclonal antibodies to specifically target and detect the α-gliadin component of wheat and homologous proteins in barley (hordein) and rye (secalin).
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Gluten ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable Almond allergen resistant to food processing effects
- Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
- Perform well in a wide variety of food products with negligible food matrix interference
