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Chloramphenicol is a broad spectrum antibiotic which is predominantly active against the main pathogen gram negative bacteria occurring in a number of food producing animals. Chloramphenicol is an antibiotic not authorised for use in food-producing animals in the European Union. Antibiotic residues such as chloramphenicol in foods pose a serious threat to public health due to aplastic anaemia. Due to this risk the use of chloramphenicol is food producing animals is prohibited in many countries worldwide with zero tolerance limits being imposed. It is therefore essential that the presence of this drug is monitored in food products such as meat, milk, honey and seafood.