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Chloramphenicol is a broad spectrum antibiotic primarily effective against gram-negative bacteria in various food-producing animals. This antibiotic is not authorised for use in food-producing animals in the European Union and many other countries worldwide due to serious public health risks, including aplastic anaemia. Antibiotic residues, such as chloramphenicol in foods, can accumulate in meat, milk, honey, and seafood, posing potential health hazards to consumers. Regulatory authorities have therefore imposed zero tolerance limits, making monitoring and detection of chloramphenicol residues essential to ensure food safety, compliance with international standards, and protection of human and animal health.