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Mustard Seed ELISA

The Mustard ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative or qualitative detection of mustard seed. This monoclonal antibody (MAb)-based assay provides a highly sensitive and specific method for the quantification of mustard presence/contamination within a variety of food matrices. It may also be used for simple “yes-no” qualitative assessments. The kit enables a facile and quantitative measure of mustard without the cross-reactivity issues often associated with polyclonal antibody (PAb)-based ELISA kits. The target indicator protein is a major mustard seed allergen and was selected based on its strong resistance to food processing and high abundance in the mustard seed.

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Description

Mustard Seed ELISA Ordering Information

Cat Code. Method Assay Time Limit of Detection (LoD) Sample Types
MS1-EK-96

MS1-EK-48

ELISA 96T

ELISA 48T

30 mins (post extraction) 0.13ppm Soups

Salad Dressings

Condiments

Cheeses

Meat

Spices

• Target major food allergens
• Robust assay with high specificity to target allergen
• Quantitative assay in 30 minutes
• Uniform extraction for multiple allergens
• Wide quantitative range (e.g., 1 – 40 ppm)
• Reduce food matrix interference and cross-reactivity
• Sensitivity at or below 1 ppm
• Ready-to-use Standards

MonoTrace ELISA kits represent the first comprehensive line of monoclonal antibody-based assays aimed at helping the food industry to accurately detect and quantify trace amounts of nut allergens in various food matrices. These ELISAs incorporate precisely defined sets
of monoclonal antibodies targeting major food allergens such as almond and provide quantitative results in just 30minutes post-extraction thanks to three 10-minute incubations!
• 10-minute sample incubation
• 10-minute conjugate incubation
• 10-minute substrate incubation

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Mustard ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

  • Target a highly stable Mustard allergen resistant to food processing effects
  • Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of ‘false positive’ results
  • Perform well in a wide variety of food products with negligible food matrix interference