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Sulfonamides are a group of synthetic organic compounds that have played an important role as effective chemotherapeutics in bacterial infections and protozoal infections in veterinary medicine. There is concern over antibiotic resistance to these drugs in the human immune system, therefore regulation of sulfonamide use needs to be monitored. In order to protect consumers, maximum residue limits (MRLs) and tolerance levels have been set in many countries. In the EU, Canada, and the USA, the limit for total sulfonamide concentration is set at less than 100 ppb in edible tissues.