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Diastase

Phadebas® Honey Diastase Test is a method for the quantitative assay of α-amylase in all honey types. Determination of the diastatic activity of honey utilising a photometric
method in which a water-insoluble, cross-linked starch polymer carrying a blue dye is used as a substrate. The substrate is hydrolysed by α-amylase, yielding blue water-soluble fragments,
determined photometrically at 620 nm. The absorbance of the solution is directly proportional to the diastatic activity of the sample. The method is based on that originally published by Siegenthaler (1) and modified by Bogdanov (2).

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Description

Assay Time Sensitivity Linearity 
– mins This method will accurately measure levels down to: The test is linear up to:

Additional information

Catalogue number

BXEFC03A – 50 Tests
BXEFC03B – 500 Tests

Method

Colorimetric