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Phadebas® Honey Diastase Test is a method for the quantitative assay of α-amylase in all honey types. Determination of the diastatic activity of honey utilising a photometric
method in which a water-insoluble, cross-linked starch polymer carrying a blue dye is used as a substrate. The substrate is hydrolysed by α-amylase, yielding blue water-soluble fragments,
determined photometrically at 620 nm. The absorbance of the solution is directly proportional to the diastatic activity of the sample. The method is based on that originally published by Siegenthaler (1) and modified by Bogdanov (2).