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Tylosin is a macrolide antibiotic and is composed primarily of Tylosin A (90%) but also minor compounds Tylosin B, C, and D. In veterinary medicine Tylosin is typically used to treat respiratory disease complexes and bacterial infections in farm animals. The powder form of Tylosin is not FDA approved for use in companion animals but it is common practice for veterinarians to prescribe this medication as oral administration and is licensed for the treatment of infections caused by organisms susceptible to the drug. Tylosin has also been used as a treatment of colitis in small animals and as a growth promotor in food-producing animals in pigs.

Tylosin maximum residue limits in Europe are currently entered in Annex III of Council Regulation (EEC) No 2377/90 are; Muscle, liver, fat, kidney 100ug/kg, milk 50ug/kg and eggs 200ug/kg. In Europe, the CRL (community reference laboratories) released a guidance document for maximum residue limits for Tylosin in honey, a recommended concentration of 20ug/kg should be achieved by any analytical method.